Adansonia Digitata Causes Redox-Sensitive Endothelium-Dependent Relaxation Involving NO in Porcine Coronary Artery

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M. Sene M. O. Kane M. Sene F. S. Barboza A. K. Sow M. Diaw F. Ba M. Sarr A. S. Diallo

Abstract

The aim of this study was to investigate the vasorelaxant effect of hydro-ethanol bark extract of Adansonia digitata (ADE) in porcine coronary arteries and to revealed mechanism of this effect. ADE has produced 100 % relaxation at 100 µg/ml dose in endothelium intact arteries pre-contracted by U46619. This effect was abolished by mechanical removal of endothelium. Different inhibitors were used to investigate the mechanism of vasorelaxation. L-nitro-arginin (L-NA) an inhibitor of endothelial NO synthase; MnTMPyP, an inhibitor of intracellular production reactive oxygen species and Wortmannin, an inhibitor of redox-sensitive pathway PI3 kinase/Akt Src has significantly reduced vasorelaxant effect of ADE whereas indomethacin (INDO), cyclooxygenase inhibitor; the apamin (APA) an inhibitor of small conductance potassium channels calcium-dependent (SKCa) and TRAM an inhibitor of intermediary conductance potassium channels calcium-dependent (IKCa) have no effect on vasorelaxation produced by ADE. Bradykinin was used to verify the presence of a functional endothelium. The results have shown that the ADE induces a redox-sensitive endothelium-dependent relaxation mediated by NO whereas prostacyclins and endothelium-derived hyperpolarizing factors (EDHF) have not appeared to play a role in the vascular effects. Hence, Adansonia digitata induces vasodilation which explain its antihypertensive effect and its use in traditional African medicine.

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How to Cite
SENE, M. et al. Adansonia Digitata Causes Redox-Sensitive Endothelium-Dependent Relaxation Involving NO in Porcine Coronary Artery. Journal of Physiology and Pharmacology Advances, [S.l.], v. 6, n. 6, p. 880-886, june 2016. ISSN 2251-7693. Available at: <http://grjournals.com/index.php/JPPA/article/view/579>. Date accessed: 18 feb. 2018.
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